Measurement

Part:BBa_K315038:Design

Designed by: Nitya Rao   Group: iGEM10_Davidson-MissouriW   (2010-07-21)


pBad + RBS + cre + pTet + lox5171 forward + RBS + RFP + lox2272 forward


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1595
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1281
    Illegal AgeI site found at 2961
    Illegal AgeI site found at 3073
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Cre recombinase is behind a weaker promoter to prevent the protein from excising all of the RFP genes in the cell. However, RFP is behind a stronger promoter because a minimal level of protein production is needed for it to be a useful reporter.


Source

This part was created in our lab.

References